Seidel et al. Fluorescence Quenching and Fluorescence Resonance Energy Transfer. The extent of quenching depends on the nature of the quencher molecule (fluorophore or non-fluorophore), the type of interaction, and the wavelength of energy that is emitted by the fluor. TYPES OF QUENCHING Concentration quenching: At low concentration linearity is observed. This may occur due to various factors like pH, concentration, temperature, viscosity, presence of oxygen, heavy metals or, specific chemical substances etc. This is a trivial type of quenching which contains little molecular information. Methods of fluorescent quenching include fluorescence resonance energy transfer (FRET), collision quenching and contact quenching, which are diagrammed below. 2 3. Fig: Quenching of quinine fluorescence in presence of chloride ions This type of complex is called static quenching and it can be described with the following equations: The order of quenching efficiency is G
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